A simple, rapid reverse phase high performance liquid chromatographic method (RP-HPLC) has been developed and validated for simultaneous estimation of Drospirenone and Ethinylestradiol in tablet dosage form. Chromatographic separation was carried out on Shimadzu HPLC (LC-20AT) using C-18 (250 mm × 4.6 mm, 5.0 μ) as stationary phase and mobile phase containing Acetonitrile:Water (60:40 v/v) at flow rate of 1 ml/min using UV detection at 225 nm. The retention time for Ethinylestradiol and Drospirenone was found to be 5.69 and 6.79 min respectively. The method was validated as per ICH guideline and successfully used for the quantitative analysis of commercially available tablet. The calibration curve was linear over the concentration range of 5-15 µg/ml for Ethinylestradiol and 50-150 µg/ml for Drospirenone. Limit of detection and Limit of quantitation was found to be 0.585 µg/ml and 1.772µg/ml for Drospirenone and 0.057 µg/ml and 0.174 µg/ml for Ethinylestradiol respectively. The % RSD below 2.0 shows the high precision of proposed method.
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